T-cell receptor sequencing reveals selected donor-reactive CD8⁺ T cell clones resist antithymocyte globulin depletion after kidney transplantation.

ABSTRACT: High frequencies of donor-reactive memory T cells in the periphery of transplant candidates prior to transplantation are linked to the development of posttransplant acute rejection episodes and reduced allograft function. Rabbit antithymocyte globulin (rATG) effectively depletes naïve CD4⁺ and CD8⁺ T cells for >6 months posttransplant, but rATG's effects on human donor-reactive T cells have not been carefully determined. To address this, we performed T cell receptor β-chain sequencing on peripheral blood mononuclear cells aliquots collected pretransplant and serially posttransplant in 7 kidney transplant recipients who received rATG as induction therapy. We tracked the evolution of the donor-reactive CD4⁺ and CD8⁺ T cell repertoires and identifie

SUBMITTER: Ningoo M 

PROVIDER: S-EPMC11070313 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature