Characterization of the malignant cells and microenvironment of infantile fibrosarcoma via single-cell RNA sequencing.

ABSTRACT:

Background

Infantile fibrosarcoma (IFS) is the most prevalent soft tissue sarcoma in children under 1 year old and is known for its rapid growth. The tumor lacks specific immunohistochemical tumor marker and a general view of tumor microenvironment (TME). Its primary therapeutic intervention places patients at a risk of disability or mutilation. This study aimed to elucidate the universal transcriptional characteristics of IFS and explore novel targets for diagnosis and therapy using single-cell RNA sequencing (scRNA-seq).

Methods

Fresh tissue samples of IFS for scRNA-seq were collected from four patients before other treatments were administered. We conducted cell clustering, inferring copy number variation from scRNA-seq (InferCNV) analysis, gene differential expression an

SUBMITTER: Li Y 

PROVIDER: S-EPMC11071021 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature