NRN1 interacts with Notch to increase oncogenic STAT3 signaling in melanoma.

ABSTRACT:

Background

Melanoma is a highly heterogeneous cancer, in which frequent changes in activation of signaling pathways lead to a high adaptability to ever changing tumor microenvironments. The elucidation of cancer specific signaling pathways is of great importance, as demonstrated by the inhibitor of the common BrafV600E mutation PLX4032 in melanoma treatment. We therefore investigated signaling pathways that were influenced by neurotrophin NRN1, which has been shown to be upregulated in melanoma.

Methods

Using a cell culture model system with an NRN1 overexpression, we investigated the influence of NRN1 on melanoma cells' functionality and signaling. We employed real time cell analysis and spheroid formation assays, while for investigation of molecular mechanisms we used a ki

SUBMITTER: Devitt L 

PROVIDER: S-EPMC11071257 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature