Measuring PETase enzyme kinetics by single-molecule microscopy.

ABSTRACT: Polyethylene terephthalate (PET) is one of the most widely produced man-made polymers and is a significant contributor to microplastics pollution. The environmental and human health impacts of microplastics pollution have motivated a concerted effort to develop microbe- and enzyme-based strategies to degrade PET and similar plastics. A PETase derived from the bacteria Ideonella sakaiensis was previously shown to enzymatically degrade PET, triggering multidisciplinary efforts to improve the robustness and activity of this and other PETases. However, because these enzymes only erode the surface of the insoluble PET substrate, it is difficult to measure standard kinetic parameters, such as k_on, k_off and kcat, complicating interpretation of the activity of mutants

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11071475 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature