Multiple direct and indirect roles of Paf1C in elongation, splicing, and histone post-translational modifications.

ABSTRACT: Paf1C is a highly conserved protein complex with critical functions during eukaryotic transcription. Previous studies have shown that Paf1C is multi-functional, controlling specific aspects of transcription, ranging from RNAPII processivity to histone modifications. However, it is unclear how specific Paf1C subunits directly impact transcription and coupled processes. We have compared conditional depletion to steady-state deletion for each Paf1C subunit to determine the direct and indirect contributions to gene expression in Saccharomyces cerevisiae. Using nascent transcript sequencing, RNAPII profiling, and modeling of transcription elongation dynamics, we have demonstrated direct effects of Paf1C subunits on RNAPII processivity and elongation rate and indirect effects on transcrip

SUBMITTER: Francette AM 

PROVIDER: S-EPMC11071476 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature