An integrated single-nucleus and spatial transcriptomics atlas reveals the molecular landscape of the human hippocampus.

ABSTRACT: The hippocampus contains many unique cell types, which serve the structure's specialized functions, including learning, memory and cognition. These cells have distinct spatial organization, morphology, physiology, and connectivity, highlighting the importance of transcriptome-wide profiling strategies that retain cytoarchitectural organization. Here, we generated spatially-resolved transcriptomics (SRT) and single-nucleus RNA-sequencing (snRNA-seq) data from adjacent tissue sections of the anterior human hippocampus in ten adult neurotypical donors to define molecular profiles for hippocampal cell types and spatial domains. Using non-negative matrix factorization (NMF) and label transfer, we integrated these data by defining gene expression patterns within the snRNA-seq data and inferring

SUBMITTER: Thompson JR 

PROVIDER: S-EPMC11071618 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature