PA28γ-20S proteasome is a proteolytic complex committed to degrade unfolded proteins.

ABSTRACT: PA28γ is a nuclear activator of the 20S proteasome that, unlike the 19S regulatory particle, stimulates hydrolysis of several substrates in an ATP- and ubiquitin-independent manner and whose exact biological functions and molecular mechanism of action still remain elusive. In an effort to shed light on these important issues, we investigated the stimulatory effect of PA28γ on the hydrolysis of different fluorogenic peptides and folded or denatured full-length proteins by the 20S proteasome. Importantly, PA28γ was found to dramatically enhance breakdown rates by 20S proteasomes of several naturally or artificially unstructured proteins, but not of their native, folded counterparts. Furthermore, these data were corroborated by experiments in cell lines with a nucleus-tagged myelin basic prot

SUBMITTER: Frayssinhes JA 

PROVIDER: S-EPMC11071804 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature