Nucleotides in both donor and acceptor splice sites are responsible for choice in NAGNAG tandem splice sites.

ABSTRACT: Among alternative splicing events in the human transcriptome, tandem NAGNAG acceptor splice sites represent an appreciable proportion. Both proximal and distal NAG can be used to produce two splicing isoforms differing by three nucleotides. In some cases, the upstream exon can be alternatively spliced as well, which further increases the number of possible transcripts. In this study, we showed that NAG choice in tandem splice site depends considerably not only on the concerned acceptor, but also on the upstream donor splice site sequence. Using an extensive set of experiments with systematically modified two-exonic minigene systems of AFAP1L2 or CSTD gene, we recognized the third and fifth intronic upstream donor splice site position and the tandem acceptor splice site region spanning from

SUBMITTER: Hujova P 

PROVIDER: S-EPMC11072513 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature