HIF-1α-activated TMEM237 promotes hepatocellular carcinoma progression via the NPHP1/Pyk2/ERK pathway.

ABSTRACT:

Background

Hypoxia-inducible factors (HIFs) are the most essential endogenous transcription factors in the hypoxic microenvironment and regulate multiple genes involved in the proliferation, migration, invasion, and EMT of hepatocellular carcinoma (HCC) cells. However, the regulatory mechanism of HIFs in driving HCC progression remains poorly understood.

Methods

Gain- and loss-of-function experiments were carried out to investigate the role of TMEM237 in vitro and in vivo. The molecular mechanisms involved in HIF-1α-induced TMEM237 expression and TMEM237-mediated enhancement of HCC progression were confirmed by luciferase reporter, ChIP, IP-MS and Co-IP assays.

Results

TMEM237 was identified as a novel hypoxia-responsive gene in HCC. HIF-1α directly bound to the promo

SUBMITTER: Chen T 

PROVIDER: S-EPMC11072547 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature