Impaired regulation of PMCA activity by defective CFTR expression promotes epithelial cell damage in alcoholic pancreatitis and hepatitis.

ABSTRACT: Alcoholic pancreatitis and hepatitis are frequent, potentially lethal diseases with limited treatment options. Our previous study reported that the expression of CFTR Cl^- channel is impaired by ethanol in pancreatic ductal cells leading to more severe alcohol-induced pancreatitis. In addition to determining epithelial ion secretion, CFTR has multiple interactions with other proteins, which may influence intracellular Ca²⁺ signaling. Thus, we aimed to investigate the impact of ethanol-mediated CFTR damage on intracellular Ca²⁺ homeostasis in pancreatic ductal epithelial cells and cholangiocytes. Human and mouse pancreas and liver samples and organoids were used to study ion secretion, intracellular signaling, protein expression and interaction. The effect of

SUBMITTER: Madacsy T 

PROVIDER: S-EPMC11073305 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature