Major depression favors the expansion of Th17-like cells and decrease the proportion of CD39⁺Treg cell subsets in response to myelin antigen in multiple sclerosis patients.

ABSTRACT:

Background

Mood disorders have been associated with risk of clinical relapses in multiple sclerosis (MS), a demyelinating disease mediated by myelin-specific T cells.

Objectives

We aimed to investigate the impact of major depressive disorder (MDD) and cytokine profile of T-cells in relapsing remitting MS patients.

Methods

For our study, plasma and PBMC were obtained from 60 MS patients (30 with lifetime MDD) in remission phase. The PBMC cultures were stimulated with anti-CD3/anti-CD28 beads or myelin basic protein (MBP), and effector and regulatory T cell phenotypes were determined by flow cytometry. The cytokine levels, both in the plasma or in the supernatants collected from PBMC cultures, were quantified by Luminex. In some experiments, the effect of serotonin (5-H

SUBMITTER: do Sacramento PM 

PROVIDER: S-EPMC11073410 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature