Investigating the mechanism of rough phenotype in a naturally attenuated Brucella strain: insights from whole genome sequencing.

ABSTRACT:

Objective

Brucellosis, a significant zoonotic disease, not only impacts animal health but also profoundly influences the host immune responses through gut microbiome. Our research focuses on whole genome sequencing and comparative genomic analysis of these Brucella strains to understand the mechanisms of their virulence changes that may deepen our comprehension of the host immune dysregulation.

Methods

The Brucella melitensis strain CMCC55210 and its naturally attenuated variant CMCC55210a were used as models. Biochemical identification tests and in vivo experiments in mice verified the characteristics of the strain. To understand the mechanism of attenuation, we then performed de novo sequencing of these two strains.

Results

We discovered

SUBMITTER: Han W 

PROVIDER: S-EPMC11073494 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature