Epigenetic Regulation of Cardiomyocyte Maturation by Arginine Methyltransferase CARM1.

ABSTRACT:

Background

During the neonatal stage, the cardiomyocyte undergoes a constellation of molecular, cytoarchitectural, and functional changes known collectively as cardiomyocyte maturation to increase myocardial contractility and cardiac output. Despite the importance of cardiomyocyte maturation, the molecular mechanisms governing this critical process remain largely unexplored.

Methods

We leveraged an in vivo mosaic knockout system to characterize the role of Carm1, the founding member of protein arginine methyltransferase, in cardiomyocyte maturation. Using a battery of assays, including immunohistochemistry, immuno-electron microscopy imaging, and action potential recording, we assessed the effect of loss of Carm1 function on cardiomyocyte cell growth, myofibril

SUBMITTER: Garbutt TA 

PROVIDER: S-EPMC11073921 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature