The transcription factor OCT6 promotes the dissolution of the naive pluripotent state by repressing Nanog and activating a formative state gene regulatory network.

ABSTRACT: In the mouse embryo, the transition from the preimplantation to the postimplantation epiblast is governed by changes in the gene regulatory network (GRN) that lead to transcriptional, epigenetic, and functional changes. This transition can be faithfully recapitulated in vitro by the differentiation of mouse embryonic stem cells (mESCs) to epiblast-like cells (EpiLCs), that reside in naïve and formative states of pluripotency, respectively. However, the GRN that drives this conversion is not fully elucidated. Here we demonstrate that the transcription factor OCT6 is a key driver of this process. Firstly, we show that Oct6 is not expressed in mESCs but is rapidly induced as cells exit the naïve pluripotent state. By deleting Oct6 in mESCs, we find that knockout cells fail to acquire the typi

SUBMITTER: Waisman A 

PROVIDER: S-EPMC11074312 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature