Truncated protein isoforms generate diversity of protein localization and function in yeast.

ABSTRACT: Genome-wide measurement of ribosome occupancy on mRNAs has enabled empirical identification of translated regions, but high-confidence detection of coding regions that overlap annotated coding regions has remained challenging. Here, we report a sensitive and robust algorithm that revealed the translation of 388 N-terminally truncated proteins in budding yeast-more than 30-fold more than previously known. We extensively experimentally validated them and defined two classes. The first class lacks large portions of the annotated protein and tends to be produced from a truncated transcript. We show that two such cases, Yap5^truncation and Pus1^truncation, have condition-specific regulation and distinct functions from their respective annotated isoforms. The second class of

SUBMITTER: Higdon AL 

PROVIDER: S-EPMC11075746 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature