An unexpected role of EasD_af: catalyzing the conversion of chanoclavine aldehyde to chanoclavine acid.

ABSTRACT: Ergot alkaloids (EAs) are a diverse group of indole alkaloids known for their complex structures, significant pharmacological effects, and toxicity to plants. The biosynthesis of these compounds begins with chanoclavine-I aldehyde (CC aldehyde, 2), an important intermediate produced by the enzyme EasD_af or its counterpart FgaDH from chanoclavine-I (CC, 1). However, how CC aldehyde 2 is converted to chanoclavine-I acid (CC acid, 3), first isolated from Ipomoea violacea several decades ago, is still unclear. In this study, we provide in vitro biochemical evidence showing that EasD_af not only converts CC 1 to CC aldehyde 2 but also directly transforms CC 1 into CC acid 3 through two sequential oxidations. Molecular docking and site-directed mutagenesis experiments confir

SUBMITTER: Yu ZP 

PROVIDER: S-EPMC11076337 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature