CArG binding factor A (CBF-A) is involved in transcriptional regulation of the rat Ha-ras promoter.

ABSTRACT: In the present study we identified a positive transcriptional element within the rat Ha-ras promoter previously known as Ha-ras response element (HRE) and identified a trans-acting factor that binds HRE sequences in rat mammary cells. To identify the binding protein we employed sequence specific DNA affinity chromatography. Amino acid sequence analysis of the affinity-purified proteins was performed by tandem mass spectroscopy. The results unexpectedly demonstrated that in rat mammary cells CArG box-binding factor A (CBF-A) is the major protein species that bind specifically to the rat and human HRE sequences with high affinity. The affinity of CBF-A binding to HRE was significantly higher than to the CArG box described as a recognition sequence for CBF-A protein. Transient transfection as

SUBMITTER: Mikheev AM 

PROVIDER: S-EPMC110773 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature