Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases.

ABSTRACT: DNA methylation is important in cellular, developmental and disease processes, as well as in bacterial restriction-modification systems. Methylation of DNA at the amino groups of cytosine and adenine is a common mode of protection against restriction endonucleases afforded by the bacterial methyltransferases. The first structure of an N:6-adenine methyltransferase belonging to the beta class of bacterial methyltransferases is described here. The structure of M. RSR:I from Rhodobacter sphaeroides, which methylates the second adenine of the GAATTC sequence, was determined to 1.75 A resolution using X-ray crystallography. Like other methyltransferases, the enzyme contains the methylase fold and has well-defined substrate binding pockets. The catalytic core most closely resembles the PVU:II me

SUBMITTER: Scavetta RD 

PROVIDER: S-EPMC110776 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature