Sterile sentinels and MinION sequencing capture active soil microbial communities that differentiate crop rotations.

ABSTRACT:

Background

Soil microbial communities are difficult to measure and critical to soil processes. The bulk soil microbiome is highly diverse and spatially heterogeneous, which can make it difficult to detect and monitor the responses of microbial communities to differences or changes in management, such as different crop rotations in agricultural research. Sampling a subset of actively growing microbes should promote monitoring how soil microbial communities respond to management by reducing the variation contributed by high microbial spatial and temporal heterogeneity and less active microbes. We tested an in-growth bag method using sterilized soil in root-excluding mesh, "sterile sentinels," for the capacity to differentiate between crop rotations. We assessed the utility of differe

SUBMITTER: Erlandson SR 

PROVIDER: S-EPMC11077770 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature