Localisation of the DmCdc45 DNA replication factor in the mitotic cycle and during chorion gene amplification.

ABSTRACT: The cdc45 protein was originally identified in Saccharomyces cerevisiae and shown to be essential for initiation of eukaryotic DNA replication. Subsequent isolation and characterisation of the corresponding genes from fission yeast, Xenopus and mammals also support a replication role for the protein in these species. They further suggest that during the course of its function cdc45 interacts with a number of other replication proteins, including minichromosome maintenance proteins, the origin recognition complex and DNA polymerase alpha. We have cloned the gene coding for cdc45 protein from Drosophila melanogaster. We have analysed the expression pattern of the cdc45 protein throughout the cell cycle and the life cycle using a combination of indirect immunofluorescence and subcellular frac

SUBMITTER: Loebel D 

PROVIDER: S-EPMC110785 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature