Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo.

ABSTRACT: Three-dimensional organoid culture technologies have revolutionized cancer research by allowing for more realistic and scalable reproductions of both tumour and microenvironmental structures^1-3. This has enabled better modelling of low-complexity cancer cell behaviours that occur over relatively short periods of time⁴. However, available organoid systems do not capture the intricate evolutionary process of cancer development in terms of tissue architecture, cell diversity, homeostasis and lifespan. As a consequence, oncogenesis and tumour formation studies are not possible in vitro and instead require the extensive use of animal models, which provide limited spatiotemporal resolution of cellular dynamics and come at a considerable cost in terms of resources and animal

SUBMITTER: Lorenzo-Martin LF 

PROVIDER: S-EPMC11078756 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature