Antibiotic-induced microbiome depletion promotes intestinal colonization by Campylobacter jejuni in mice.

ABSTRACT:

Background

To establish a method to induce Campylobacter jejuni colonization in the intestines of C57BL/6 mice through antibiotic-induced microbiome depletion.

Results

Fifty-four female C57BL/6 mice were divided into the normal, control, and experimental groups. The experimental group was administered intragastric cefoperazone sodium and sulbactam sodium (50 mg/mL) for 2 days; then, the experimental and control mice were intragastrically administered 200 µL C. jejuni, which was repeated once more after 2 days. Animal feces were collected, and the HipO gene of C. jejuni was detected using TaqMan qPCR from day 1 to day 14 after modeling completion. Immunofluorescence was used to detect intestinal C. jejuni colonization on day 14, and pathological changes were observed using he

SUBMITTER: Chen H 

PROVIDER: S-EPMC11080253 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature