Lysosomal BK channels facilitate silica-induced inflammation in macrophages.

ABSTRACT:

Background

Lysosomal ion channels are proposed therapeutic targets for a number of diseases, including those driven by NLRP3 inflammasome-mediated inflammation. Here, the specific role of the lysosomal big conductance Ca²⁺-activated K⁺ (BK) channel was evaluated in a silica model of inflammation in murine macrophages. A specific-inhibitor of BK channel function, paxilline (PAX), and activators NS11021 and NS1619 were utilized to evaluate the role of lysosomal BK channel activity in silica-induced lysosomal membrane permeabilization (LMP) and NLRP3 inflammasome activation resulting in IL-1β release.

Methods

Murine macrophages were exposed in vitro to crystalline silica following pretreatment with BK channel inhibitors or activators and LMP, cell d

SUBMITTER: Kendall RL 

PROVIDER: S-EPMC11080613 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature