Age-Dependent RGS5 Loss in Pericytes Induces Cardiac Dysfunction and Fibrosis.

ABSTRACT:

Background

Pericytes are capillary-associated mural cells involved in the maintenance and stability of the vascular network. Although aging is one of the main risk factors for cardiovascular disease, the consequences of aging on cardiac pericytes are unknown.

Methods

In this study, we have combined single-nucleus RNA sequencing and histological analysis to determine the effects of aging on cardiac pericytes. Furthermore, we have conducted in vivo and in vitro analysis of RGS5 (regulator of G-protein signaling 5) loss of function and finally have performed pericytes-fibroblasts coculture studies to understand the effect of RGS5 deletion in pericytes on the neighboring fibroblasts.

Results

Aging reduced the pericyte area and capillary coverage in the murine heart. Singl

SUBMITTER: Tamiato A 

PROVIDER: S-EPMC11081481 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature