Structural and functional insights into transcription activation of the essential LysR-type transcriptional regulators.

ABSTRACT: The enormous LysR-type transcriptional regulators (LTTRs), which are diversely distributed amongst prokaryotes, play crucial roles in transcription regulation of genes involved in basic metabolic pathways, virulence and stress resistance. However, the precise transcription activation mechanism of these genes by LTTRs remains to be explored. Here, we determine the cryo-EM structure of a LTTR-dependent transcription activation complex comprising of Escherichia coli RNA polymerase (RNAP), an essential LTTR protein GcvA and its cognate promoter DNA. Structural analysis shows two N-terminal DNA binding domains of GcvA (GcvA_DBD) dimerize and engage the GcvA activation binding sites, presenting the -35 element for specific recognition with the conserved σ⁷⁰R4. In particular, the versa

SUBMITTER: Shi J 

PROVIDER: S-EPMC11081524 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature