Super-resolution proximity labeling with enhanced direct identification of biotinylation sites.

ABSTRACT: Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein-protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemented, in-depth identification of specific biotinylation sites remains challenging, and current approaches are technically demanding with low yields. A novel method to systematically identify specific biotinylation sites for LC-MS analysis followed by proximity labeling showed excellent performance compared with that of related approaches in terms of identification depth with high enrichment power. The systematic identification of biotinylation sites enabled a simpler and more efficient experimental design to identify subcellular localized pro

SUBMITTER: Shin S 

PROVIDER: S-EPMC11082246 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature