Systematic Exploration of Optimized Base Editing gRNA Design and Pleiotropic Effects with BExplorer.

ABSTRACT: Base editing technology is being increasingly applied in genome engineering, but the current strategy for designing guide RNAs (gRNAs) relies substantially on empirical experience rather than a dependable and efficient in silico design. Furthermore, the pleiotropic effect of base editing on disease treatment remains unexplored, which prevents its further clinical usage. Here, we presented BExplorer, an integrated and comprehensive computational pipeline to optimize the design of gRNAs for 26 existing types of base editors in silico. Using BExplorer, we described its results for two types of mainstream base editors, BE3 and ABE7.10, and evaluated the pleiotropic effects of the corresponding base editing loci. BExplorer revealed 524 and 900 editable pathogenic single nucleotide polymorphism

SUBMITTER: Zhang G 

PROVIDER: S-EPMC11082405 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature