RNA-Seq Bulked Segregant Analysis of an Exotic B. napus ssp. napobrassica (Rutabaga) F₂ Population Reveals Novel QTLs for Breeding Clubroot-Resistant Canola.

ABSTRACT: In this study, a rutabaga (Brassica napus ssp. napobrassica) donor parent FGRA106, which exhibited broad-spectrum resistance to 17 isolates representing 16 pathotypes of Plasmodiophora brassicae, was used in genetic crosses with the susceptible spring-type canola (B. napus ssp. napus) accession FG769. The F₂ plants derived from a clubroot-resistant F₁ plant were screened against three P. brassicae isolates representing pathotypes 3A, 3D, and 3H. Chi-square (χ²) goodness-of-fit tests indicated that the F₂ plants inherited two major clubroot resistance genes from the CR donor FGRA106. The total RNA from plants resistant (R) and susceptible (S) to each pathotype were pooled and subjected to bulked segregant

SUBMITTER: Yu Z 

PROVIDER: S-EPMC11083300 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature