LINC01021 Attenuates Expression and Affects Alternative Splicing of a Subset of p53-Regulated Genes.

ABSTRACT:

Background

Loss of the p53-inducible LINC01021 in p53-proficient CRC cell lines results in increased sensitivity to DNA-damaging chemotherapeutics. Here, we comprehensively analyze how LINC01021 affects the p53-induced transcriptional program.

Methods

Using a CRISPR/Cas9-approach, we deleted the p53 binding site in the LINC01021 promoter of SW480 colorectal cancer cells and subjected them to RNA-Seq analysis after the activation of ectopic p53. RNA affinity purification followed by mass spectrometry was used to identify proteins associated with LINC01021.

Results

Loss of the p53-inducibility of LINC01021 resulted in an ~1.8-fold increase in the number of significantly regulated mRNAs compared to LINC01021 wild-type cells after

SUBMITTER: Kaller M 

PROVIDER: S-EPMC11083319 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature