Susceptibility of Melanoma Cells to Targeted Therapy Correlates with Protection by Blood Neutrophils.

ABSTRACT: Elevated levels of peripheral blood and tumor tissue neutrophils are associated with poorer clinical response and therapy resistance in melanoma. The underlying mechanism and the role of neutrophils in targeted therapy is still not fully understood. Serum samples of patients with advanced melanoma were collected and neutrophil-associated serum markers were measured and correlated with response to targeted therapy. Blood neutrophils from healthy donors and patients with advanced melanoma were isolated, and their phenotypes, as well as their in vitro functions, were compared. In vitro functional tests were conducted through nonadherent cocultures with melanoma cells. Protection of melanoma cell lines by neutrophils was assessed under MAPK inhibition. Blood neutrophils from advanced melanoma

SUBMITTER: Wendlinger S 

PROVIDER: S-EPMC11083732 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature