The nature of chronic rejection after lung transplantation: a murine orthotopic lung transplant study.

ABSTRACT:

Introduction

Chronic rejection is a major complication post-transplantation. Within lung transplantation, chronic rejection was considered as airway centred. Chronic Lung Allograft Dysfunction (CLAD), defined to cover all late chronic complications, makes it more difficult to understand chronic rejection from an immunological perspective. This study investigated the true nature, timing and location of chronic rejection as a whole, within mouse lung transplantation.

Methods

40 mice underwent an orthotopic left lung transplantation, were sacrificed at day 70 and evaluated by histology and in vivo µCT. For timing and location of rejection, extra grafts were sacrificed at day 7, 35, 56 and investigated by ex vivo µCT or single cell RNA (scRNA) profiling.

Results

Chronic r

SUBMITTER: Heigl T 

PROVIDER: S-EPMC11084670 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature