Senescent characteristics of human corneal endothelial cells upon ultraviolet-A exposure.

ABSTRACT:

Purpose

The objective of this study was to investigate the senescent phenotypes of human corneal endothelial cells (hCEnCs) upon treatment with ultraviolet (UV)-A.

Methods

We assessed cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, cell proliferation and expression of senescence markers (p16 and p21) in hCEnCs exposed to UV-A radiation, and senescent hCEnCs induced by ionizing radiation (IR) were used as positive controls. We performed RNA sequencing and proteomics analyses to compare gene and protein expression profiles between UV-A- and IR-induced senescent hCEnCs, and we also compared the results to non-senescent hCEnCs.

Results

Cells exposed to 5 J/cm2 of UV-A or to IR exhibited typical senescent phenotypes, including enla

SUBMITTER: Numa K 

PROVIDER: S-EPMC11087119 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature