DELLA proteins recruit the Mediator complex subunit MED15 to coactivate transcription in land plants.

ABSTRACT: DELLA proteins are negative regulators of the gibberellin response pathway in angiosperms, acting as central hubs that interact with hundreds of transcription factors (TFs) and regulators to modulate their activities. While the mechanism of TF sequestration by DELLAs to prevent DNA binding to downstream targets has been extensively documented, the mechanism that allows them to act as coactivators remains to be understood. Here, we demonstrate that DELLAs directly recruit the Mediator complex to specific loci in Arabidopsis, facilitating transcription. This recruitment involves DELLA amino-terminal domain and the conserved MED15 KIX domain. Accordingly, partial loss of MED15 function mainly disrupted processes known to rely on DELLA coactivation capacity, including cytokinin-dependent regul

SUBMITTER: Hernandez-Garcia J 

PROVIDER: S-EPMC11087773 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature