Engineered polymer nanoparticles as artificial chaperones facilitating the selective refolding of denatured enzymes.

ABSTRACT: Molecular chaperones assist in protein refolding by selectively binding to proteins in their nonnative states. Despite progress in creating artificial chaperones, these designs often have a limited range of substrates they can work with. In this paper, we present molecularly imprinted flexible polymer nanoparticles (nanoMIPs) designed as customizable biomimetic chaperones. We used model proteins such as cytochrome c, laccase, and lipase to screen polymeric monomers and identify the most effective formulations, offering tunable charge and hydrophobic properties. Utilizing a dispersed phase imprinting approach, we employed magnetic beads modified with destabilized whole-protein as solid-phase templates. This process involves medium exchange facilitated by magnetic pulldowns, resulting in the

SUBMITTER: Li Y 

PROVIDER: S-EPMC11087784 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature