Chemotherapy-induced PTEN-L secretion promotes the selection of PTEN-deficient tumor cells.

ABSTRACT:

Background

PTEN loss has been identified in various tumor types and is linked to unfavorable clinical outcomes. In addition to PTEN mutation, multiple mechanisms contribute to PTEN loss during tumor development. However, the natural selection process of PTEN-deficient tumor cells remains unclear. Here, we aimed at further elucidating the role of PTEN-L in tumor progression.

Methods

PTEN knockout cell lines were generated using CRISPR/Cas9 technology. Ni-NTA affinity column chromatography was employed for PTEN-L purification. Tumor cell metastasis was evaluated in murine models and observed using the IVIS Spectrum Imaging System. RNA-sequencing, western blotting, PCR, flow cytometry, and cell proliferation assays were employed to investigate tumor cell dormancy and related me

SUBMITTER: Wang M 

PROVIDER: S-EPMC11088029 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature