Single-cell transcriptomic analysis of decidual immune cell landscape in the occurrence of adverse pregnancy outcomes induced by Toxoplasma gondii infection.

ABSTRACT:

Background

Toxoplasma gondii is an obligate intracellular parasite that can lead to adverse pregnancy outcomes, particularly in early pregnancy. Previous studies have illustrated the landscape of decidual immune cells. However, the landscape of decidual immune cells in the maternal-fetal microenvironment during T. gondii infection remains unknown.

Methods

In this study, we employed single-cell RNA sequencing to analyze the changes in human decidual immune cells following T. gondii infection. The results of scRNA-seq were further validated with flow cytometry, reverse transcription-polymerase chain reaction, western blot, and immunofluorescence staining.

Results

Our results showed that the proportion of 17 decidual immune cell clusters and the expression levels of 21 g

SUBMITTER: Fu T 

PROVIDER: S-EPMC11088043 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature