Identification and validation of stable reference genes for RT-qPCR analyses of Kobresia littledalei seedlings.

ABSTRACT:

Background

Kobreisa littledalei, belonging to the Cyperaceae family is the first Kobresia species with a reference genome and the most dominant species in Qinghai-Tibet Plateau alpine meadows. It has several resistance genes which could be used to breed improved crop varieties. Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) is a popular and accurate gene expression analysis method. Its reliability depends on the expression levels of reference genes, which vary by species, tissues and environments. However, K.littledalei lacks a stable and normalized reference gene for RT-qPCR analysis.

Results

The stability of 13 potential reference genes was tested and the stable reference genes were selected for RT-qPCR normalization for the expression ana

SUBMITTER: Sun H 

PROVIDER: S-EPMC11088182 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature