Biocatalytic strategy for the construction of sp³-rich polycyclic compounds from directed evolution and computational modelling.

ABSTRACT: Catalysis with engineered enzymes has provided more efficient routes for the production of active pharmaceutical agents. However, the potential of biocatalysis to assist in early-stage drug discovery campaigns remains largely untapped. In this study, we have developed a biocatalytic strategy for the construction of sp³-rich polycyclic compounds via the intramolecular cyclopropanation of benzothiophenes and related heterocycles. Two carbene transferases with complementary regioisomer selectivity were evolved to catalyse the stereoselective cyclization of benzothiophene substrates bearing diazo ester groups at the C2 or C3 position of the heterocycle. The detailed mechanisms of these reactions were elucidated by a combination of crystallographic and computational analyses. Leverag

SUBMITTER: Vargas DA 

PROVIDER: S-EPMC11088497 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature