Rapid in vivo multiplexed editing (RIME) of the adult mouse liver.

ABSTRACT:

Background and aims

Assessing mammalian gene function in vivo has traditionally relied on manipulation of the mouse genome in embryonic stem cells or perizygotic embryos. These approaches are time-consuming and require extensive breeding when simultaneous mutations in multiple genes is desired. The aim of this study is to introduce a rapid in vivo multiplexed editing (RIME) method and provide proof of concept of this system.

Approach and results

RIME, a system wherein CRISPR/caspase 9 technology, paired with adeno-associated viruses (AAVs), permits the inactivation of one or more genes in the adult mouse liver. The method is quick, requiring as little as 1 month from conceptualization to knockout, and highly efficient, enabling editing in >95% of target cells. To highlight i

SUBMITTER: Katsuda T 

PROVIDER: S-EPMC11088813 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature