Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding.

ABSTRACT: Learning and memory require activity-induced changes in dendritic translation, but which mRNAs are involved and how they are regulated are unclear. In this study, to monitor how depolarization impacts local dendritic biology, we employed a dendritically targeted proximity labeling approach followed by crosslinking immunoprecipitation, ribosome profiling and mass spectrometry. Depolarization of primary cortical neurons with KCl or the glutamate agonist DHPG caused rapid reprogramming of dendritic protein expression, where changes in dendritic mRNAs and proteins are weakly correlated. For a subset of pre-localized messages, depolarization increased the translation of upstream open reading frames (uORFs) and their downstream coding sequences, enabling localized production of proteins involved

SUBMITTER: Hacisuleyman E 

PROVIDER: S-EPMC11088998 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature