Immunometric and functional measurement of endogenous vasoinhibin in human sera.

ABSTRACT:

Introduction

Circulating levels of the antiangiogenic protein vasoinhibin, a fragment of prolactin, are of interest in vasoproliferative retinopathies, preeclampsia, and peripartum cardiomyopathy; however, it is difficult to determine the circulating levels of vasoinhibin due to the lack of quantitative assays.

Methods

This study used human serum samples to assess the concentration and bioactivity of vasoinhibin using a novel enzyme-linked immunosorbent assay (ELISA) for human vasoinhibin, which employs an anti-vasoinhibin monoclonal antibody, a human umbilical vein endothelial cell (HUVEC) proliferation assay, and a chick chorioallantoic membrane (CAM) angiogenesis assay.

Results

Serum samples from 17 pregnant women without (one group) and with preeclampsia and pregn

SUBMITTER: Zamora M 

PROVIDER: S-EPMC11089174 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature