Acacetin alleviates autoimmune myocarditis by regulating CD4+ T cell mitochondrial respiration.

ABSTRACT:

Background

Myocarditis refers to an autoimmune inflammatory response of the myocardium with characterization of self-reactive CD4+ T cell activation, which lacks effective treatment and has a poor prognosis. Acacetin is a natural flavonoid product that has been reported to have anti-inflammatory effects. However, acacetin has not been investigated in myocarditis.

Methods

Oral acacetin treatment was administered in an experimental autoimmune myocarditis model established with myosin heavy chain-alpha peptide. Echocardiography, pathological staining, and RT-qPCR were used to detect cardiac function, myocardial injury, and inflammation levels. Flow cytometry was utilized to detect the effect of acacetin on CD4+ T cell function. RNA-seq, molecular docking, and microscale thermop

SUBMITTER: Lu Y 

PROVIDER: S-EPMC11089761 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature