Universal Digital High-Resolution Melt Analysis for the Diagnosis of Bacteremia.

ABSTRACT: Fast and accurate diagnosis of bloodstream infection is necessary to inform treatment decisions for septic patients, who face hourly increases in mortality risk. Blood culture remains the gold standard test but typically requires approximately 15 hours to detect the presence of a pathogen. We, therefore, assessed the potential for universal digital high-resolution melt (U-dHRM) analysis to accomplish faster broad-based bacterial detection, load quantification, and species-level identification directly from whole blood. Analytical validation studies demonstrated strong agreement between U-dHRM load measurement and quantitative blood culture, indicating that U-dHRM detection is highly specific to intact organisms. In a pilot clinical study of 17 whole blood samples from pediatric patients un

SUBMITTER: Aralar A 

PROVIDER: S-EPMC11090205 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature