Defining albumin as a glycoprotein with multiple N-linked glycosylation sites.

ABSTRACT:

Background

Glycosylation is an enzyme-catalyzed post-translational modification that is distinct from glycation and is present on a majority of plasma proteins. N-glycosylation occurs on asparagine residues predominantly within canonical N-glycosylation motifs (Asn-X-Ser/Thr) although non-canonical N-glycosylation motifs Asn-X-Cys/Val have also been reported. Albumin is the most abundant protein in plasma whose glycation is well-studied in diabetes mellitus. However, albumin has long been considered a non-glycosylated protein due to absence of canonical motifs. Albumin contains two non-canonical N-glycosylation motifs, of which one was recently reported to be glycosylated.

Methods

We enriched abundant serum proteins to investigate their N-linked glycosylation followed by try

SUBMITTER: Garapati K 

PROVIDER: S-EPMC11090807 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature