Development of PCR-based markers for identification of wheat HMW glutenin Glu-1Bx and Glu-1By alleles.

ABSTRACT:

Background

In common wheat (Triticum aestivum L.), allelic variations in the high-molecular-weight glutenin subunits Glu-B1 locus have important effects on grain end-use quality. The Glu-B1 locus consists of two tightly linked genes encoding x- and y-type subunits that exhibit highly variable frequencies. However, studies on the discriminating markers of the alleles that have been reported are limited. Here, we developed 11 agarose gel-based PCR markers for detecting Glu-1Bx and Glu-1By alleles.

Results

By integrating the newly developed markers with previously published PCR markers, nine Glu-1Bx locus alleles (Glu-1Bx6, Glu-1Bx7, Glu-1Bx7*, Glu-1Bx7 ^OE, Glu-1Bx13, Glu-1Bx14 ^(-) , Glu-1Bx14 ⁽⁺⁾/Bx20, and Glu-1Bx17) and seven Glu-1By locus all

SUBMITTER: Lee MH 

PROVIDER: S-EPMC11092038 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature