Nanopore and Illumina sequencing reveal different viral populations from human gut samples.

ABSTRACT: The advent of viral metagenomics, or viromics, has improved our knowledge and understanding of global viral diversity. High-throughput sequencing technologies enable explorations of the ecological roles, contributions to host metabolism, and the influence of viruses in various environments, including the human intestinal microbiome. However, bacterial metagenomic studies frequently have the advantage. The adoption of advanced technologies like long-read sequencing has the potential to be transformative in refining viromics and metagenomics. Here, we examined the effectiveness of long-read and hybrid sequencing by comparing Illumina short-read and Oxford Nanopore Technology (ONT) long-read sequencing technologies and different assembly strategies on recovering viral genomes from human faeca

SUBMITTER: Cook R 

PROVIDER: S-EPMC11092197 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature