Deep profiling of potential substrate atlas of porcine epidemic diarrhea virus 3C-like protease.

ABSTRACT: Coronavirus (CoV) 3C-like protease (3CL^pro) is essential for viral replication and is involved in immune escape by proteolyzing host proteins. Deep profiling the 3CL^pro substrates in the host proteome extends our understanding of viral pathogenesis and facilitates antiviral drug discovery. Here, 3CL^pro from porcine epidemic diarrhea virus (PEDV), an enteropathogenic CoV, was used as a model which to identify the potential 3CL^pro cleavage motifs in all porcine proteins. We characterized the selectivity of PEDV 3CL^pro at sites P5-P4'. We then compiled the 3CL^pro substrate preferences into a position-specific scoring matrix and developed a 3CL^pro profiling strategy to delineate the protein substrate landscape of CoV

SUBMITTER: Zhou J 

PROVIDER: S-EPMC11092332 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature