Control of 3' splice site selection by the yeast splicing factor Fyv6.

ABSTRACT: Pre-mRNA splicing is catalyzed in two steps: 5' splice site (SS) cleavage and exon ligation. A number of proteins transiently associate with spliceosomes to specifically impact these steps (1^st and 2^nd step factors). We recently identified Fyv6 (FAM192A in humans) as a 2^nd step factor in S. cerevisiae; however, we did not determine how widespread Fyv6's impact is on the transcriptome. To answer this question, we have used RNA-seq to analyze changes in splicing. These results show that loss of Fyv6 results in activation of non-consensus, branch point (BP) proximal 3' SS transcriptome-wide. To identify the molecular basis of these observations, we determined a high-resolution cryo-EM structure of a yeast product complex spliceosome containing Fyv6 at 2

SUBMITTER: Senn KA 

PROVIDER: S-EPMC11092753 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature