Toward a MALDI in-source decay (ISD) method for top-down analysis of protein footprinting.

ABSTRACT: Irreversible protein footprinting is a mass spectrometry-based approach in which solvent-accessible sites of a protein are modified to assess high-order protein structure. Structural insights can be gained by determining the position and extents of modification. The usual approach to obtain the "footprint" is to analyze the protein through bottom-up LC-MS/MS. In this approach, the proteins are digested to yield a mixture of peptides that are then separated by LC before locating the modification sites by MS/MS. This process consumes substantial amounts of time and is difficult to accelerate for applications that require quick and high-throughput analysis. Here, we describe employing matrix-assisted laser desorption/ionization (MALDI) in-source decay (ISD) to analyze a footprinted small test

SUBMITTER: Jiang R 

PROVIDER: S-EPMC11092977 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature