METTL8 links mt-tRNA m³C modification to the HIF1α/RTK/Akt axis to sustain GBM stemness and tumorigenicity.

ABSTRACT: Epitranscriptomic RNA modifications are crucial for the maintenance of glioma stem cells (GSCs), the most malignant cells in glioblastoma (GBM). 3-methylcytosine (m³C) is a new epitranscriptomic mark on RNAs and METTL8 represents an m³C writer that is dysregulated in cancer. Although METTL8 has an established function in mitochondrial tRNA (mt-tRNA) m³C modification, alternative splicing of METTL8 can also generate isoforms that localize to the nucleolus where they may regulate R-loop formation. The molecular basis for METTL8 dysregulation in GBM, and which METTL8 isoform(s) may influence GBM cell fate and malignancy remain elusive. Here, we investigated the role of METTL8 in regulating GBM stemness and tumorigenicity. In GSC, METTL8 is exclusively localize

SUBMITTER: Lee BWL 

PROVIDER: S-EPMC11093979 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature